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KIT 抗体 (Internal Region)

This anti-KIT antibody is a 山羊 多克隆 antibody detecting KIT in WB, FACS, ELISA, IHC 和 IF. Suitable for 人.
产品编号 ABIN1589992
发货至: 中国

Quick Overview for KIT 抗体 (Internal Region) (ABIN1589992)

抗原

See all KIT 抗体
KIT (Mast/stem Cell Growth Factor Receptor (KIT))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This KIT antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    KIT / CD117

    序列

    KRDSFICSKQ EDH

    特异性

    This antibody is expected to recognize both reported isoforms (NP_000213.1, NP_001087241.1).

    交叉反应

    Cow, 犬, 人, Pig

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 110 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110 kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incub

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended co

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    KIT (Mast/stem Cell Growth Factor Receptor (KIT))

    别名

    KIT

    背景

    KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian

    基因ID

    3815

    NCBI登录号

    NP_000213, NP_001087241

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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