GREM1 抗体
Quick Overview for GREM1 抗体 (ABIN1589921)
抗原
See all GREM1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Gremlin-1 antibody
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特异性
- Recombinant mouse Gremlin-1
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产品特性
- Chromosomal location: 2 E4, 2 64.5 cM
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纯化方法
- Protein A purified
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免疫原
- Recombinant mouse Gremlin-1 (ABIN1589733)
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亚型
- IgG
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anti-Gremlin 1 (GREM1) (AA 101-184) antibody
GREM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 147-175), (C-Term) antibodyGREM1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB2060 unconjugated
anti-Gremlin 1 (GREM1) (AA 26-184) antibodyGREM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 25-184) antibodyGREM1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 26-184) antibodyGREM1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Gremlin 1 (GREM1) (AA 25-184) antibodyGREM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 75-184) antibodyGREM1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D1 unconjugated
anti-Gremlin 1 (GREM1) (AA 18-184) antibodyGREM1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 27-184) antibodyGREM1 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gremlin 1 (GREM1) (AA 147-175), (C-Term) antibodyGREM1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: use at 2-5 μg/mL, FACS: use at 3-10 μg/mL, IF/IHC: applicable with cryo-sections.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- GREM1 (Gremlin 1 (GREM1))
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别名
- Gremlin-1
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背景
- Grem1, ld, Drm, Cktsf1b1,Gremlin, also known as ""Increased in High Glucose protein 2"" (IHG2) and ""Down regulated in Mos-transformed cells protein"" (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Native human Gremlin consist of 160 amino acids. The mature region contains one potential site for N-linked glycosylation (Asn42), a cysteine-rich region, and a cysteine-knot motif (aa94-184) whose structure is shared by members of the TGFβ superfamily. Posttranslational modifications include glycosylation and phosphorylation. Human Gremlin exists in both secreted and membrane-associated forms and there exist 2 isoforms. The aa sequence identity of human Gremlin with mouse and chicken Gremlin is 99 % and 86 %, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle. Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP2, BMP4, and BMP7, thus preventing them from interacting with their cell surface receptors. This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development and to play a role in human diseases, such as diabetic nephropathy. However, intracellular BMP-independent mechanisms of action may mediate the ability of Gremlin to suppress transformation and tumor genesis under certain experimental conditions. Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis. In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium. Furthermore Gremlin is a novel agonist of the major proangiogenic receptor VEGFR2.
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基因ID
- 23892
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NCBI登录号
- NM_011824, NP_035954
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UniProt
- O70327
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途径
- Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location
抗原
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