PLGF 抗体 (N-Term)
Quick Overview for PLGF 抗体 (N-Term) (ABIN1589873)
抗原
See all PLGF (PGF) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- PlGF (Peptide) antibody
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特异性
- N-terminal peptide (20aa) of native human PlGF
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产品特性
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Chromosomal location: 2p21-p16
Produced from sera of rabbits immunized with a highly purified N-terminal peptide of human PlGF (placenta growth factor). -
纯化方法
- Anti-PlGF was purified by affinity chromatography with immobilized Protein A.
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免疫原
- N-terminal peptide (20aa) of native human PlGF
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亚型
- IgG
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anti-Placenta Growth Factor (PGF) (Internal Region) antibody
PGF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 166-221) antibodyPGF 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 19-170) antibodyPGF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 101-158) antibodyPGF 适用: 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 43-162) antibodyPGF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 24-158) antibodyPGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) antibodyPGF 适用: 人 ELISA, IHC 宿主: 兔 Monoclonal 1B6 unconjugated Recombinant Antibody
anti-Placenta Growth Factor (PGF) (AA 1-170) antibodyPGF 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 1-170) antibodyPGF 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Placenta Growth Factor (PGF) (AA 131-158) antibodyPGF 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: Use 2-10 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- PLGF (PGF) (Placenta Growth Factor (PGF))
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别名
- PlGF
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背景
- PlGF, placental growth factor,Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56 % , 55 %, 74 % and 95 % aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.
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基因ID
- 5281
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NCBI登录号
- NM_001207012, NP_001193941
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UniProt
- P49763
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途径
- VEGFR1 Specific Signals
抗原
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