PDGFC 抗体
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北京 101111
Quick Overview for PDGFC 抗体 (ABIN1589843)
抗原
See all PDGFC 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- VEGF-E antibody
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特异性
- Recombinant ov-VEGF-E
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产品特性
- Produced from sera of rabbits immunized with highly pure recombinant ov-VEGF-E produced in E. coli. A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor -2/KDR.
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纯化方法
- Protein A purified
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免疫原
- Recombinant ov-VEGF-E (ABIN1589587)
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亚型
- IgG
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anti-Platelet-Derived Growth Factor C (PDGFC) antibody
PDGFC 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 201-300) antibodyPDGFC 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 235-345) antibodyPDGFC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) antibodyPDGFC 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 236-345) antibodyPDGFC 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 1-345) antibodyPDGFC 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 23-345) antibodyPDGFC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 74-103) antibodyPDGFC 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 166-345) antibodyPDGFC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet-Derived Growth Factor C (PDGFC) (AA 235-345) antibodyPDGFC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: Use 1-5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
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别名
- VEGF-E
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背景
- VEGF-E,A DNA sequence encoding the mature variant of ov-VEGF-E isolate D1701 was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight Cysteine residues of the central Cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E cannot bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor -2/KDR.
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UniProt
- Q9YMF3
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途径
- RTK signaling, Platelet-derived growth Factor Receptor Signaling
抗原
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