FLT4 抗体 (Extracellular Domain) (Biotin)
Quick Overview for FLT4 抗体 (Extracellular Domain) (Biotin) (ABIN1589800)
抗原
See all FLT4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- VEGFR-3/FLT-4 antibody
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特异性
- Recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain
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交叉反应 （详细）
- The unconjugated antibody will detect native human VEGFR-3/FLT-4 in ELISA experiments.
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产品特性
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Chromosomal location: 5q35.3
The antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain. VEGFR-3/FLT-4 mediates the angiogenic activity of VEGF-C and VEGF-D on lymphatic endothelial cells. -
纯化方法
- The IgG1 fraction of the hybridoma supernatant was purified by Protein G affinity chromatography and then biotinylated using a standard protocol. VEGFR-3/FLT-4, belongs to the class III subfamily of receptor tyrosine kinases.
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免疫原
- recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain
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亚型
- IgG1
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anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 25-259) antibody
FLT4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS, ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1174-1363) antibodyFLT4 适用: 人 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1-439) antibodyFLT4 适用: 人 WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 818CT12-1-1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 818CT12-1-1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 901-1000) antibodyFLT4 适用: 人, 小鼠, Cow ELISA, FACS, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 720-760) antibodyFLT4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 34-133) antibodyFLT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5B5 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) antibodyFLT4 适用: 人 IHC (p) 宿主: 小鼠 Monoclonal 8C1 unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 640-700) antibodyFLT4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1112-1329) antibodyFLT4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: Use at 2-5 μg/mL, ELISA: Use at 1-10 μg/mL, IP: use at 2-10 μg/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, BSA (50x), 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))
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别名
- VEGFR-3/FLT-4
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背景
- Vascular endothelial growth factor receptor-3, FLT4, PCL, LMPH1A, VEGFR3, fms-related tyrosine kinase 4,The recombinant mature sVEGFR-3/FLT-4 is a glycosylated monomeric protein. The sVEGFR-3/FLT-4 monomers have a mass of approximately 120 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis.
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基因ID
- 2324
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NCBI登录号
- NM_002020
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UniProt
- P35916
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途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
抗原
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