TIE1 抗体 (Biotin)
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北京 101111
Quick Overview for TIE1 抗体 (Biotin) (ABIN1589694)
抗原
See all TIE1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- TIE-1 antibody
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特异性
- Recombinant human soluble extracellular TIE-1
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交叉反应 （详细）
- The unconjugated antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.
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产品特性
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Chromosomal location: 1p34-p33
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen. The unconjugated antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting. -
纯化方法
- Mouse IgG1 antibody (#6F12) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.
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免疫原
- Recombinant human soluble extracellular TIE-1
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亚型
- IgG1
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anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 385-607) antibody
TIE1 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8D12D2 unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 385-607) antibodyTIE1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 8D12B10 unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 435-609) antibodyTIE1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 701-898) antibodyTIE1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 701-898) antibodyTIE1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 217-328) antibodyTIE1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) antibodyTIE1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB725 unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 536-643) antibodyTIE1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) antibodyTIE1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 361-389) antibodyTIE1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: Use at 1-15 μg/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, BSA (50x), 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Centrifuge vial prior to opening.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))
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别名
- TIE-1
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背景
- TIE1, TIE, JTK14,Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
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基因ID
- 7075
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NCBI登录号
- NM_005424, NP_005415
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UniProt
- P35590
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途径
- RTK signaling
抗原
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