Western Blotting (WB), ELISA, Flow Cytometry (FACS)
原理
TIE-1 antibody
特异性
Recombinant human soluble TIE-2
交叉反应 (详细)
The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.
产品特性
Chromosomal location: 1p34-p33 The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen.
纯化方法
Mouse IgG1 antibody (#6F12) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
ELISA: Use at 1-15 μg/mL, Western blotting: Use at 1-2 μg/mL, FACS analysis and cell sorting: Test under progress.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS
注意事项
Centrifuge vial prior to opening.
储存条件
4 °C,-20 °C
储存方法
The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
有效期
24 months
抗原
TIE1
(tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))
TIE1, TIE, JTK14,Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). It is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2.