For immunofluorescence, use MCA-DA2 diluted 1/500. For western blots try MCA-DA2 diluted 1/10,000 to 1:20,000.
限制
仅限研究用
状态
Liquid
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 4°C short term or -20°C long term.
抗原
NEFL
(Neurofilament, Light Polypeptide (NEFL))
别名
Neurofilament NF-L
背景
Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. This antibody has been sold by Chemicon and Millipore for many years under the catalog number Mab1615, and has been used in numerous publications. The same antibody is also marketed, more expensively, by Abcam, Novus and many other companies.