NEFH 抗体
Quick Overview for NEFH 抗体 (ABIN1580444)
抗原
See all NEFH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- ascites fluid
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亚型
- IgG2b
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应用备注
- The ascites solution has a high titre and can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
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限制
- 仅限研究用
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状态
- Liquid
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4°C short term or -20°C long term.
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- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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背景
- Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. MCA-9B12 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with MCA-9B12 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.
抗原
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