Protein A 抗体 (Biotin)
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- 抗原 See all Protein A 抗体
- Protein A
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适用
- Staphylococcus aureus
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Protein A antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB)
- 特异性
- The Protein A Antibody [Biotin], mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuRe M ligand.
- 产品特性
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This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A. The Protein A Antibody [Biotin], mAb, Mouse is Protein A Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of Protein A.
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. - 纯化方法
- Protein A affinity column
- 免疫原
- purified recombinant Protein A
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product Protein A Primary Antibody
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anti-Protein A antibody (HRP)
适用: Staphylococcus aureus ELISA, WB 宿主: 小鸡 Polyclonal HRP
anti-Protein A antibody适用: Staphylococcus aureus IP, Con 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, FM 宿主: 山羊 Polyclonal Texas Red (TR)
anti-Protein A antibody (HRP)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal HRP
anti-Protein A antibody (FITC)适用: Staphylococcus aureus FACS 宿主: 山羊 Polyclonal FITC
anti-Protein A antibody (Biotin)适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal Biotin
anti-Protein A antibody (Texas Red (TR))适用: Staphylococcus aureus FACS, IM 宿主: 山羊 Polyclonal Texas Red (TR)
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- 应用备注
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Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/mL ELISA Detection: 0.05-0.2 µg/mL Western Blot: 0.5 -1 µg/mL Other applications: user-optimized - 限制
- 仅限研究用
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- 溶解方式
- Reconstitute the lyophilized product with 200 µl deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- -20 °C
- 储存方法
- The Protein A Antibody [Biotin], mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
- 有效期
- 12 months
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- 抗原
- Protein A
- Abstract
- Protein A 产品
- 别名
- alpha protein antibody, repB antibody
- 背景
- Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in sample foods or for evaluation of Protein A resin.
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