tau 抗体 (pThr217)
Quick Overview for tau 抗体 (pThr217) (ABIN1573963)
抗原
See all tau 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr217
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特异性
- Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody detects endogenous levels of Tau only when phosphorylated at threonine 217.
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纯化方法
- The antibody is purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.
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免疫原
- Synthetic phospho-peptide (KLH-coupled) corresponding - AA surrounding pThr217 of human Tau
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亚型
- IgG
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anti-tau Protein (pSer396) antibody
适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-tau Protein (AA 677-707) antibody适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-tau Protein (AA 681-758) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-tau Protein antibody适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 12F4 unconjugated
anti-tau Protein (pSer202), (pThr205) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-tau Protein (AA 12-24), (pTyr18) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal 9G3 unconjugated
anti-tau Protein antibody适用: 人 WB, ELISA, IF, ICC, DB 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-tau Protein antibody (HRP)适用: 人 WB, ELISA, IF, ICC, DB 宿主: 小鼠 Monoclonal 1D5 HRP
anti-tau Protein antibody (FITC)适用: 人 WB, ELISA, IF, ICC, DB 宿主: 小鼠 Monoclonal 1D5 FITC
anti-tau Protein antibody (Biotin)适用: 人 WB, ELISA, IF, ICC, DB 宿主: 小鼠 Monoclonal 1D5 Biotin
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, pH 7.4, containing 30 % glycerol and 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- tau (tau Protein)
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别名
- tau
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背景
- Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal part.Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer-disease (AD). These PHFs are also found in the lesions of other central nervous system disorders.Rabbit Anti-Tau (Phospho-Thr217) Polyclonal Antibody is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr217 of human Tau.
抗原
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