Prostate Specific Antigen 抗体
(1 validation)Quick Overview for Prostate Specific Antigen 抗体 (ABIN1543584)
抗原
See all Prostate Specific Antigen (PSA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity column
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免疫原
- Native PSA Antigen
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亚型
- IgG1
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anti-Prostate Specific Antigen (PSA) (AA 1-100) antibody
PSA 适用: 人 IHC, WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Prostate Specific Antigen (PSA) (AA 26-251) antibodyPSA 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5A11E2 unconjugated
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal A67-B-E3 unconjugated
anti-Prostate Specific Antigen (PSA) (AA 39-261) antibodyPSA 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人 IHC, WB, IHC (p), FACS, ICC, IHC (fro), ELISpot 宿主: 小鼠 Monoclonal C3F12 unconjugated
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人 IHC, StM, ISt 宿主: 兔 Monoclonal KLK3-2871R unconjugated Recombinant Antibody
anti-Prostate Specific Antigen (PSA) (AA 150-250) antibodyPSA 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-603R unconjugated Recombinant Antibody
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人, 兔 ELISA, WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal Pa3 unconjugated
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal PA5 unconjugated
anti-Prostate Specific Antigen (PSA) antibodyPSA 适用: 人, Cat ELISA, WB, IHC (p), FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal 1A7 unconjugated
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说明
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Other Applications have not been tested.
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限制
- 仅限研究用
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- by
- Flow Cytometry & Cell Separation Facility, Purdue University
- No.
- #029617
- 日期
- 2014.02.22
- 抗原
- Lot Number
- FAPS-A5D5-0003
- Method validated
- Flow Cytometry
- Positive Control
- LNCaP cells
- Negative Control
- - DU145 cells - Jurkat cells
- Notes
- Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
- Primary Antibody
- Antibody: Prostate Specific Antigen (PSA)
- Catalog number: ABIN1543584
- Lot number: FAPS-A5D5-0003
- Secondary Antibody
- Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
- Full Protocol
- Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
- Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
- An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
- Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
- Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
- Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
- 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
- Cells were washed twice with PBS/BSA/Tween 20
- Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
- Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
- Cells were washed twice with PBS/BSA/Tween 20
- Cells were resuspended in 0.3 mL PBS/BSA
- Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
- Experimental Notes
- None
生效 #029617 (Flow Cytometry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).]()
Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).
![Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).]()
Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).
![Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).]()
Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).
Full Methods -
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状态
- Liquid
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浓度
- 1.8 mg/mL
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缓冲液
- PBS, pH 7.4, 0.09% sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- Prostate Specific Antigen (PSA)
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别名
- PSA
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途径
- Complement System
抗原
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