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MELK 抗体 (AA 431-480)

The 兔 多克隆 anti-MELK antibody is suitable to detect MELK in samples from 人, 小鼠 和 大鼠. It has been validated for WB, ELISA, IHC 和 IF.
产品编号 ABIN1533892
发货至: 中国
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Quick Overview for MELK 抗体 (AA 431-480) (ABIN1533892)

抗原

See all MELK 抗体
MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

适用

  • 74
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  • 4
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  • 2
  • 2
人, 小鼠, 大鼠

宿主

  • 73
  • 6
兔

克隆类型

  • 71
  • 8
多克隆

标记

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  • 1
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  • 1
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This MELK antibody is un-conjugated

应用范围

  • 42
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  • 9
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  • 4
  • 3
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  • 1
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

    • 15
    • 13
    • 8
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
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    • 1
    • 1
    • 1
    • 1
    AA 431-480

    原理

    MELK Antibody

    特异性

    MELK Polyclonal Antibody detects endogenous levels of MELK protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human MELK. AA range:431-480

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

    别名

    MELK

    背景

    Synonyms: MELK, KIAA0175, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK

    Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.,PTM:Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with ZNF622 and PPP1R8.,tissue specificity:Expressed in placenta, kidney, thymus, testis, ovary and intestine.,

    Gene Name: MELK

    Protein Name: Maternal embryonic leucine zipper kinase

    分子量

    74 kDa

    基因ID

    9833

    OMIM

    607025

    UniProt

    Q14680
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