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WEE1 抗体 (pSer53)

The 兔 多克隆 anti-WEE1 antibody is suitable to detect WEE1 in samples from 人, 小鼠 和 大鼠. It has been validated for ELISA, IHC 和 IF.
产品编号 ABIN1532086
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for WEE1 抗体 (pSer53) (ABIN1532086)

抗原

See all WEE1 抗体
WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

适用

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人, 小鼠, 大鼠

宿主

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兔

克隆类型

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多克隆

标记

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This WEE1 antibody is un-conjugated

应用范围

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ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

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    AA 19-68, pSer53

    原理

    Wee 1 (phospho Ser53) Antibody

    特异性

    Phospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53. AA range:19-68

    亚型

    IgG
  • 应用备注

    IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

    别名

    WEE1

    背景

    Synonyms: WEE1, Wee1-like protein kinase, WEE1hu, Wee1A kinase

    Background: WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],

    Gene Name: WEE1

    Protein Name: Wee1-like protein kinase

    分子量

    71 kDa

    基因ID

    7465

    OMIM

    193525

    UniProt

    P30291

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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