H2AFX 抗体 (pSer139)
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北京 101111
Quick Overview for H2AFX 抗体 (pSer139) (ABIN1531862)
抗原
See all H2AFX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 94-143, pSer139
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原理
- Histone H2A.X (phospho Ser139) Antibody
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特异性
- Phospho-Histone H2A.X (S139) Polyclonal Antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at S139.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- H2AFX (H2A Histone Family, Member X (H2AFX))
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别名
- Histone H2A.X
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背景
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Synonyms: H2AFX, H2AX, Histone H2A.x, H2a/x
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],
Gene Name: H2AFX
Protein Name: Histone H2A.x,γH2AX
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分子量
- 15 kDa
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基因ID
- 3014
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OMIM
- 601772
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UniProt
- P16104
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途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
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