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EIF4E 抗体 (pSer209)

This 兔 多克隆 antibody specifically detects EIF4E in WB, IHC, ELISA 和 IF. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN1531825
发货至: 中国
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Quick Overview for EIF4E 抗体 (pSer209) (ABIN1531825)

抗原

See all EIF4E 抗体
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

适用

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  • 5
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人, 小鼠, 大鼠

宿主

  • 144
  • 20
  • 2
兔

克隆类型

  • 109
  • 57
多克隆

标记

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  • 1
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This EIF4E antibody is un-conjugated

应用范围

  • 138
  • 73
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  • 36
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  • 27
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

    • 53
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    • 1
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    • 1
    AA 168-217, pSer209

    原理

    eIF4E (phospho Ser209) Antibody

    特异性

    Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209. AA range:168-217

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    别名

    eIF4E

    背景

    Synonyms: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

    Background: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

    Gene Name: EIF4E

    Protein Name: Eukaryotic translation initiation factor 4E

    分子量

    25 kDa

    基因ID

    1977

    OMIM

    133440

    UniProt

    P06730

    途径

    BCR Signaling
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