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RPA2 抗体 (pThr21)

The 兔 多克隆 anti-RPA2 antibody (ABIN1531626) specifically detects RPA2 in WB, IHC, ELISA 和 IF. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN1531626
发货至: 中国
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Quick Overview for RPA2 抗体 (pThr21) (ABIN1531626)

抗原

See all RPA2 抗体
RPA2 (Replication Protein A2, 32kDa (RPA2))

适用

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人, 小鼠

宿主

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  • 48
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兔

克隆类型

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  • 95
多克隆

标记

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This RPA2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

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    AA 10-59, pThr21

    原理

    RPA32 (phospho Thr21) Antibody

    特异性

    Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    RPA2 (Replication Protein A2, 32kDa (RPA2))

    别名

    RFA2

    背景

    Synonyms: RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34

    Background: function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,

    Gene Name: RPA2

    Protein Name: Replication protein A 32 kDa subunit

    分子量

    29 kDa

    基因ID

    6118

    OMIM

    179836

    UniProt

    P15927

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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