CD28 抗体 (pTyr218)
Quick Overview for CD28 抗体 (pTyr218) (ABIN1531543)
抗原
See all CD28 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 171-220, pTyr218
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特异性
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CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
PhosphorylationH:Y218 M:Y216
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纯化方法
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
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亚型
- IgG
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应用备注
- WB: 1:500~1:1000 ELISA: 1:40000
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说明
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Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable at -20°C for at least 1 year.
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有效期
- 12 months
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- CD28
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别名
- CD28
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背景
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Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
NCBI Gene Symbol: CD28
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分子量
- 25 kDa
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基因ID
- 940
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OMIM
- 186760
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UniProt
- P10747
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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