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Histone H3.3 抗体 (pSer31)

This 兔 多克隆 antibody specifically detects Histone H3.3 in ELISA, IHC 和 IF. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN1531501
发货至: 中国
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Quick Overview for Histone H3.3 抗体 (pSer31) (ABIN1531501)

抗原

See all Histone H3.3 (H3F3A) 抗体
Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

适用

  • 100
  • 46
  • 44
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 95
  • 13
兔

克隆类型

  • 57
  • 51
多克隆

标记

  • 56
  • 7
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Histone H3.3 antibody is un-conjugated

应用范围

  • 77
  • 65
  • 37
  • 37
  • 29
  • 11
  • 8
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

    • 13
    • 6
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 16-65, pSer31

    原理

    Histone H3.3 (phospho Ser31) Antibody

    特异性

    Phospho-Histone H3.3 (S31) Polyclonal Antibody detects endogenous levels of Histone H3.3 protein only when phosphorylated at S31.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. AA range:16-65

    亚型

    IgG
  • 应用备注

    IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

    别名

    Histone H3.3

    背景

    Synonyms: H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3

    Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008],

    Gene Name: H3F3A

    Protein Name: Histone H3.3

    分子量

    15 kDa

    基因ID

    3020, 3021

    OMIM

    601058

    UniProt

    P84243
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