TK (AA 1-50), (pSer13) 抗体
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北京 101111
Quick Overview for TK (AA 1-50), (pSer13) 抗体 (ABIN1531403)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-50, pSer13
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原理
- Thymidine Kinase (phospho Ser13) Antibody
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特异性
- Phospho-Thymidine Kinase (S13) Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein only when phosphorylated at S13.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human TK around the phosphorylation site of Ser13. AA range:1-50
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- TK
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背景
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Synonyms: TK1, Thymidine kinase, cytosolic
Background: catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,
Gene Name: TK1
Protein Name: Thymidine kinase cytosolic
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分子量
- 25 kDa
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基因ID
- 7083
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OMIM
- 188300
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UniProt
- P04183
抗原
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