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AMPK alpha 抗体 (pThr172)

The 兔 多克隆 anti-AMPK alpha antibody (ABIN1531172) specifically detects AMPK alpha in WB, IHC, ELISA 和 IF. The antibody is reactive with 人, 小鼠, 大鼠, 猴, Pig 和 Shrimp samples.
产品编号 ABIN1531172
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for AMPK alpha 抗体 (pThr172) (ABIN1531172)

抗原

See all AMPK alpha (SNF1A) 抗体
AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

适用

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人, 小鼠, 大鼠, 猴, Pig, Shrimp

宿主

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兔

克隆类型

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多克隆

标记

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This AMPK alpha antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

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    AA 140-189, pThr172

    原理

    AMPKα1/2 (phospho Thr183/172) Antibody

    特异性

    Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

    亚型

    IgG
  • 应用备注

    IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

    别名

    AMPK alpha

    背景

    Synonyms: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

    Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

    Gene Name: AAPK1/AAPK2

    Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2

    分子量

    62 kDa

    基因ID

    5562, 5563

    OMIM

    602739

    UniProt

    Q13131

    途径

    Warburg Effect
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