AMPK alpha 抗体 (pThr172)
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Quick Overview for AMPK alpha 抗体 (pThr172) (ABIN1531172)
抗原
See all AMPK alpha (SNF1A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 140-189, pThr172
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原理
- AMPKα1/2 (phospho Thr183/172) Antibody
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特异性
- Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
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亚型
- IgG
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应用备注
- IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))
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别名
- AMPK alpha
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背景
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Synonyms: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Gene Name: AAPK1/AAPK2
Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2
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分子量
- 62 kDa
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基因ID
- 5562, 5563
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OMIM
- 602739
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UniProt
- Q13131
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途径
- Warburg Effect
抗原
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