Digoxigenin 抗体 (FITC)
The FITC-conjugated 小鼠 单克隆 anti-Digoxigenin antibody (Clone 21H8) (ABIN1514934) specifically detects Digoxigenin in IHC, ISH 和 FACS.
The antibody is reactive with 化学剂 samples.
Independently validated for use in Immunohistochemistry.
(1 validation)
产品编号 ABIN1514934
发货至:
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Quick Overview for Digoxigenin 抗体 (FITC) (ABIN1514934)
抗原
Digoxigenin (DIG)
适用
化学剂
宿主
小鼠
克隆类型
单克隆
标记
This Digoxigenin antibody is conjugated to FITC
应用范围
Immunohistochemistry (IHC), In situ hybridization (ISH), Flow Cytometry (FACS)
克隆位点
21H8
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特异性
- Reacts with free and bound digoxigenin
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纯化方法
- Purified (protein A)
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免疫原
- Digoxigenin
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亚型
- IgG1 kappa
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应用备注
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC-F(1:20 - 1:2000) -
限制
- 仅限研究用
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-
- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #102325
- 日期
- 2026.06.08
- 抗原
- Digoxigenin (DIG)
- Lot Number
- 17F7
- Method validated
- Immunohistochemistry
- Positive Control
Alkaline Phosphatase Anti-Digoxigenin antibody (Abcam, Catalog # ab119345).
- Negative Control
Omission of Terminal transferase.
- Notes
The antibody works in IHC-P at concentration of 1:100.
- Primary Antibody
- ABIN1514934
- Secondary Antibody
- Full Protocol
- Sample analyzed: P5 CD1 mouse brain
- Paraffin-embedded slices (7 µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
- Sections were incubated in proteinase K [20 µg/mL] for 15 minutes at room temperature (RT).
- 4 × 2 minutes washing in double distilled water (ddH2O).
- Sections were briefly washed in DN buffer and incubated in DNase [1 µg/mL] for 10 minutes at RT.
- 4 × 2 minutes washing in ddH2O.
- 5 minutes washing in TDT buffer at RT.
- Sections were incubated in TDT buffer added with Terminal transferase [0.05 U/µL] and digoxigenin-11-dUTP [10 µM] for 2 hours at +37 °C.
- 15 minutes washing in TB buffer at RT.
- 5 minutes washing in ddH2O + 5 minutes washing in 0.01 M PBS.
- Sections were incubated with the FITC-conjugated antibody at the dilution of 1:100, overnight at +4 °C (antibody was diluted in PLL/BSA/PBS diluent added with 5% BSA).
- 3 × 5 minutes washing in 0.01 M PBS.
- Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) [1 µg/mL], 2 minutes.
- 2 × 5 minutes washing in 0.01 M PBS.
- Specimens were mounted in Fluoroshield mounting medium (Sigma).
- Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 40× magnification.
- POSITIVE CONTROL: Sections from the same tissues were incubated with an anti-digoxigenin antibody conjugated with alkaline phosphatase (AP) and developed with NBT/BCIP solution.
- NEGATIVE CONTROL: Omission of Terminal transferase.
- OTHER PERTINENT REAGENTS USED:
- Proteinase K (Thermo Scientific, Catalog # EO0491)
- DNase I (in the kit by Sigma, Catalog # AMP-D1)
- Terminal transferase (in the kit by Jena Biosciences, Catalog # APP-002)
- Digoxigenin-11-dUTP (Roche, Catalog # 11573179910)
- DN buffer: Tris base [30 mM] pH 7.2, sodium cacodylate [140 mM], magnesium chloride [4 mM], DTT [0.1 mM] in dH2O
- TDT buffer: Tris base [30 mM] pH 7.2, sodium cacodylate [140 mM], cobalt chloride [1 mM] in dH2O
- TB buffer: sodium chloride [300 mM], sodium citrate [30 mM] in dH2O
- For positive control: Alkaline Phosphatase Anti-Digoxigenin antibody (Abcam, Catalog # ab119345)
- Experimental Notes
To exclude any nonspecific signal due to the secondary antibody, one control slice was processed for each experimental procedure, omitting the primary antibody.
生效 #102325 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Positive control with Alkaline Phosphatase Anti-Digoxigenin antibody on P5 CD1 mouse brain. Images show reaction with and without DNase treatment to increase cellular death. Magnification: 40x.]()
Positive control with Alkaline Phosphatase Anti-Digoxigenin antibody on P5 CD1 mouse brain. Images show reaction with and without DNase treatment to increase cellular death. Magnification: 40x.
![Digoxigenin (DIG) (FITC-conjugated) immunoreactive cells in the cerebral cortex, hippocampus and cerebellum of P5 CD1 mice. Sections were treated with DNase and then subjected to the TUNEL reaction. Magnification: 40x.]()
Digoxigenin (DIG) (FITC-conjugated) immunoreactive cells in the cerebral cortex, hippocampus and cerebellum of P5 CD1 mice. Sections were treated with DNase and then subjected to the TUNEL reaction. Magnification: 40x.
Full Methods -
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, Glycerol 50 % , BSA 1 %,( pH 7.4), Thiomersal 0.02 %
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- Digoxigenin (DIG)
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别名
- Digoxigenin
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物质类
- Chemical
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背景
- Digoxigenin (DIG) is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications.
抗原
-
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