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CKS1 抗体 (C-Term)

This 兔 多克隆 antibody specifically detects CKS1 in WB, ELISA, IF, IHC, IHC (p) 和 IP. It exhibits reactivity toward 人.
产品编号 ABIN1493317
发货至: 中国
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Quick Overview for CKS1 抗体 (C-Term) (ABIN1493317)

抗原

See all CKS1 (CKS1B) 抗体
CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

适用

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宿主

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克隆类型

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多克隆

标记

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This CKS1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • 抗原表位

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    C-Term

    特异性

    Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.

    交叉反应 (详细)

    Cross-reactivity with mouse is expected based upon amino acid sequence homology.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide derived from the C-terminal region of human and mouse Cks1.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (0.1 - 1 μg/mL), IF (10 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: 10 μg/mL. Immunoprecipitation: 7 μg/IP reaction. Immunohistochemistry: 2.5 μg/mL. Note: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epitope Retrieval (HIER) with citrate buffer,  pH 6.0, is recommended.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

    别名

    CKS1B / CKS1

    背景

    Name/Gene ID: CKS1B
    Subfamily: CKS

    Synonyms: CKS1B, CDC28 protein kinase 1, CKS-1, CDC28 protein kinase 1B, Ckshs1, PNAS-18, PNAS-143, PNAS-16, CDC2-associated protein CKS1, CKS1

    基因ID

    1163

    UniProt

    P61024

    途径

    Mitotic G1-G1/S Phases, Inositol Metabolic Process
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