GIT1 抗体 (AA 561-610)
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北京 101111
Quick Overview for GIT1 抗体 (AA 561-610) (ABIN1450893)
抗原
See all GIT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 561-610
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原理
- GIT1 Antibody
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特异性
- GIT1 Polyclonal Antibody detects endogenous levels of GIT1 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- The antiserum was produced against synthesized peptide derived from human GIT1. AA range:561-610
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亚型
- IgG
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anti-GIT1 (GIT1) (AA 460-640) antibody
KO Validated GIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 533-561), (C-Term), (Tyr554) antibodyGIT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB15351 unconjugated
anti-GIT1 (GIT1) (AA 470-642) antibodyGIT1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 415-736) antibodyGIT1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 487-517) antibodyGIT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB15373 unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibody (Atto 390)GIT1 适用: 大鼠 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal S39B-8 Atto 390
anti-GIT1 (GIT1) (AA 375-770) antibody (Atto 488)GIT1 适用: 大鼠 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal S39B-8 Atto 488
anti-GIT1 (GIT1) (AA 375-770) antibody (Atto 594)GIT1 适用: 大鼠 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal S39B-8 Atto 594
anti-GIT1 (GIT1) (AA 375-770) antibody (FITC)GIT1 适用: 大鼠 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal S39B-8 FITC
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- GIT1
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别名
- GIT1
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背景
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Synonyms: GIT1, ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1
Background: domain:The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.,function:GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.,PTM:Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 3 ANK repeats.,subcellular location:Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.,subunit:Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4. Interacts with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK (By similarity). Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1.,
Gene Name: GIT1
Protein Name: ARF GTPase-activating protein GIT1
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分子量
- 84 kDa
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基因ID
- 28964
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OMIM
- 608434
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UniProt
- Q9Y2X7
抗原
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