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GFP 抗体

This 山羊多克隆 antibody specifically detects GFP in WB, IF, IHC (fro) 和 IHC (p). It exhibits reactivity toward Aequorea victoria. It has been mentioned in 18+ publications

Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1439998) (GFP 抗体)

5 images

(18 references)

产品编号 ABIN1439998

发货至: 中国

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Quick Overview for GFP 抗体 (ABIN1439998)

抗原

See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))

适用

Aequorea victoria

宿主

山羊

克隆类型

多克隆

标记

This GFP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product GFP Primary Antibody

原理

anti-GFP

序列

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

特异性

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

产品特性

Green Fluorescent Protein

纯化方法

Epitope affinity purified

免疫原

Purified recombinant peptide produced in E. coli.

亚型

IgG
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 1-100) antibody
GFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000

限制

仅限研究用
浓度

2 mg/mL

缓冲液

PBS, 20 % glycerol and 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

The antibody solution should be gently mixed before use.

储存条件

4 °C,-20 °C

储存方法

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Panayiotou, Michael, Zaravinos, Demirag, Achilleos, Strati: "Human papillomavirus E7 binds Oct4 and regulates its activity in HPV-associated cervical cancers." in: PLoS pathogens, Vol. 16, Issue 4, pp. e1008468, (2020) (PubMed).

Wu, Yi, Manca, Javaid, Lauer, Glowatzki: "Sound exposure dynamically induces dopamine synthesis in cholinergic LOC efferents for feedback to auditory nerve fibers." in: eLife, Vol. 9, (2020) (PubMed).

Fukuda, Kaneshige, Kaji, Noguchi, Takemoto, Zhang, Tsujikawa, Kokubo, Uezumi, Maehara, Harada, Ohkawa, Fukada: "Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle." in: eLife, Vol. 8, (2020) (PubMed).

Zhang, Uezumi, Kaji, Tsujikawa, Andersen, Jensen, Fukada: "Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration." in: International journal of molecular sciences, Vol. 20, Issue 13, (2019) (PubMed).

Vyas, Wu, Jimenez, Glowatzki, Fuchs: "Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea." in: Scientific reports, Vol. 9, Issue 1, pp. 5549, (2019) (PubMed).

Larson, Mironova, Vanderpool, Waisman, Rash, Agarwal, Bergles: "Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility." in: eLife, Vol. 7, (2019) (PubMed).

Ramos-Nascimento, Kellen, Ferreira, Alenquer, Vale-Costa, Raposo, Delevoye, Amorim: "KIF13A mediates trafficking of influenza A virus ribonucleoproteins." in: Journal of cell science, Vol. 130, Issue 23, pp. 4038-4050, (2018) (PubMed).

Wu, Vyas, Glowatzki, Fuchs: "Opposing expression gradients of calcitonin-related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea." in: The Journal of comparative neurology, Vol. 526, Issue 3, pp. 425-438, (2018) (PubMed).

Festa, Chen, Berquez, Debaix, Tokonami, Prange, Hoek, Alessio, Raimondi, Nevo, Giles, Devuyst, Luciani: "Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney." in: Nature communications, Vol. 9, Issue 1, pp. 161, (2018) (PubMed).

Mimura, Takagi, Clever, Imamoto: "ELYS regulates the localization of LBR by modulating its phosphorylation state." in: Journal of cell science, Vol. 129, Issue 22, pp. 4200-4212, (2017) (PubMed).

Vale-Costa, Alenquer, Sousa, Kellen, Ramalho, Tranfield, Amorim: "Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors." in: Journal of cell science, Vol. 129, Issue 8, pp. 1697-710, (2017) (PubMed).

Agarwal, Wu, Hughes, Fukaya, Tischfield, Langseth, Wirtz, Bergles: "Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes." in: Neuron, Vol. 93, Issue 3, pp. 587-605.e7, (2017) (PubMed).

Vyas, Wu, Zimmerman, Fuchs, Glowatzki: "Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 18, Issue 1, pp. 139-151, (2017) (PubMed).

Martins-Marques, Anjo, Pereira, Manadas, Girão: "Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 11, pp. 3040-55, (2016) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." in: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Kourti, Ikonomou, Giakoumakis, Rapsomaniki, Landegren, Siniossoglou, Lygerou, Simos, Mylonis: "CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation." in: Cellular signalling, Vol. 27, Issue 6, pp. 1129-40, (2015) (PubMed).

Paukert, Agarwal, Cha, Doze, Kang, Bergles: "Norepinephrine controls astroglial responsiveness to local circuit activity." in: Neuron, Vol. 82, Issue 6, pp. 1263-70, (2014) (PubMed).

Pais, Milho, Almeida, Mota: "Identification of novel type III secretion chaperone-substrate complexes of Chlamydia trachomatis." in: PLoS ONE, Vol. 8, Issue 2, pp. e56292, (2013) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP

背景

Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Green fluorescent protein antibody.

分子量

26.9 kDa