AAV VP1 抗体 (HRP)
Quick Overview for AAV VP1 抗体 (HRP) (ABIN1406570)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
-
交叉反应
- Virus
-
交叉反应 （详细）
- AAV5
-
纯化方法
- Purified by Protein A.
-
免疫原
- KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
-
亚型
- IgG
-
-
anti-Adeno-Associated Virus, VP1 (AAV VP1) antibody
AAV VP1 适用: Adeno-Associated Virus (AAV) IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
WB 1:100-1000
IHC-P 1:100-500 -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 μg/μL
-
缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
-
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
-
有效期
- 12 months
-
-
- AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
-
别名
- Adeno-Associated Virus Capsid Protein VP1
-
物质类
- Viral Protein
-
背景
-
Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.
Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
抗原
-
