AAV VP1 抗体
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- 抗原 See all AAV VP1 products
- AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
- 适用
- Adeno-Associated Virus (AAV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AAV VP1 antibody is un-conjugated
- 应用范围
- Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 特异性
- This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
- 交叉反应
- Virus
- 交叉反应 （详细）
- AAV5
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
- 亚型
- IgG
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- 应用备注
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WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
- 别名
- Adeno-Associated Virus Capsid Protein VP1 (AAV VP1 产品)
- 物质类
- Viral Protein
- 背景
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Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.
Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
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