5-Methylcytosine 抗体 (AbBy Fluor® 647)
Quick Overview for 5-Methylcytosine 抗体 (AbBy Fluor® 647) (ABIN1402782)
抗原
适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 人
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交叉反应 （详细）
- 5 MethylCytosine
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated 5 MethylCytosine
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亚型
- IgG
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anti-5-Methylcytosine antibody
WB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine antibody适用: 人 MeDIP, IF, DB 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine antibody (Biotin)ICC, IF 宿主: 小鼠 Monoclonal 5MC-CD Biotin
anti-5-Methylcytosine antibody (FITC)ICC, IF 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine antibodyICC, IF 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyWB, MeDIP, FACS, ICC, IF, DB, IHC, ELISA 宿主: 小鼠 Monoclonal 33D3 unconjugated
anti-5-Methylcytosine antibodyMeDIP, DB, MeDIP-Seq 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine antibodyMeDIP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- IF(IHC-P) 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- 5-Methylcytosine
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别名
- 5 MethylCytosine
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物质类
- Chemical
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背景
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Synonyms: 5-Methyl Cytidine, 5 m C, 5 mC, 5 me C, 5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5-mC, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine.
Background: Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
抗原
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