PUM1 抗体 (AA 201-300)
Quick Overview for PUM1 抗体 (AA 201-300) (ABIN1387238)
抗原
See all PUM1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 201-300
-
交叉反应
- 小鼠, 大鼠
-
预测反应
- Human,Dog,Cow,Sheep,Pig,Rabbit
-
纯化方法
- Purified by Protein A.
-
免疫原
- KLH conjugated synthetic peptide derived from human Pumilio 1
-
亚型
- IgG
-
-
anti-Pumilio Homolog 1 (PUM1) (AA 1-130) antibody
PUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-280) antibodyPUM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 62-91) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB16264 unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-1186) antibodyPUM1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (Internal Region) antibodyPUM1 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (AA 1-30), (N-Term) antibodyPUM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB10535 unconjugated
anti-Pumilio Homolog 1 (PUM1) (N-Term) antibodyPUM1 适用: 人 IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 1 (PUM1) (Tyr83) antibodyPUM1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 μg/μL
-
缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
-
有效期
- 12 months
-
-
- PUM1 (Pumilio Homolog 1 (PUM1))
-
别名
- Pumilio 1
-
背景
-
Synonyms: PUMH, HSPUM, PUMH1, PUML1, Pumilio homolog 1, Pumilio-1, PUM1, KIAA0099
Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity).
-
基因ID
- 9698
-
UniProt
- Q14671
抗原
-
