EIF4E 抗体 (AA 121-217)
(1 validation)Quick Overview for EIF4E 抗体 (AA 121-217) (ABIN1386772)
抗原
See all EIF4E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 121-217
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交叉反应
- 人, 大鼠
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预测反应
- Mouse
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human eIF4E
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-100) antibody
EIF4E 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D11 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2B1 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 2-217) antibodyEIF4E 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 1F3 unconjugated Recombinant Antibody
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 -
限制
- 仅限研究用
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- by
- Immunohistochemistry Core, NYU Langone
- No.
- #029640
- 日期
- 2014.03.14
- 抗原
- Lot Number
- 140106
- Method validated
- Immunohistochemistry
- Positive Control
- Human pancreas
- Negative Control
- Human colon
- Notes
- Clear strong signal was detected in positive control sample. Note a minor amount of expected background staining in negative control sample.
- Primary Antibody
- Antigen: Eukaryotic Translation Initiation Factor 4E (EIF4E)
- Catalog number: ABIN1386772
- Lot number: 140106
- Secondary Antibody
- Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
- Lot number: D07640BA
- Full Protocol
- Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
- 1. Slides were preheated in convection oven at 60°C for 30 min
- 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
- 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
- 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
- 5. Slides rinsed with *reaction buffer* x3
- 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
- 7. Slides rinsed with *reaction buffer*
- 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
- 9. Slides rinsed with *reaction buffer* x3
- 10. *iView Biotinylated IgG* applied and incubated for 8 min
- 11. Slides rinsed with *reaction buffer*
- 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
- 15. Slides rinsed with *reaction buffer*
- 16. *iView DAB/H2O2* applied and incubated for 8 min
- 17. Slides rinsed with *reaction buffer*
- 18. *iView Copper* applied and incubated for 4 min
- 19. Slides rinsed with *reaction buffer*
- 20. Slides washed in Dawn Detergent/tap water
- 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
- 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
- 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
- Experimental Notes
- Deviations from protocol/procedure supplied by manufacturer (attached).
- Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
- Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
- Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
- Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
- Step 4: Italicized reagents and incubation time are fixed instrument parameters.
- Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
- Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
- Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
生效 #029640 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Human pancreas tissue stained with anti-EIF4E (brown) and counterstained with hematoxylin.]()
Figure 1: Human pancreas tissue stained with anti-EIF4E (brown) and counterstained with hematoxylin.
![Figure 2: Human colon tissue stained with anti-EIF4E (brown) and counterstained with hematoxylin.]()
Figure 2: Human colon tissue stained with anti-EIF4E (brown) and counterstained with hematoxylin.
![Figure 3: Human pancreas tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.]()
Figure 3: Human pancreas tissue stained with isotype control antibody (brown) and counterstained with hematoxylin.
![Figure 4: Human pancreas tissue stained with secondary antibody only (brown) and counterstained with hematoxylin.]()
Figure 4: Human pancreas tissue stained with secondary antibody only (brown) and counterstained with hematoxylin.
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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别名
- eIF4E
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背景
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Synonyms: CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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基因ID
- 1977
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UniProt
- P06730
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途径
- BCR Signaling
抗原
-
