A chicken was immunized with a peptide representing the splice junction of osteopontin-c (Ac-SEEKQNAVSC-COOH). After multiple immunizations, eggs were collected and IgY was purified from the egg yolk. The antibody was affinity purified on an osteopontin-c peptide column. No sodium azide was added.
This antibody has been tested in ELISA and a suggested starting dilution for ELISA analysis is 2 ug/ml. Optimal working dilutions should be determined for your particular assay conditions. For Western blotting, recommended concentration 1 ug/ml.