Bu-1 抗体
(2 references)Quick Overview for Bu-1 抗体 (ABIN135594)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Chicken Bu-1, Mr 70 kDa
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产品特性
- Mouse Anti-Chicken Bu-1-UNLB
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纯化方法
- Purified
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免疫原
- Chicken peripheral blood lymphocytes and splenocytes
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亚型
- IgG1
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anti-Bu-1 antibody (Biotin)
适用: 小鸡 FACS 宿主: 小鼠 Monoclonal AV20 Biotin
anti-Bu-1 antibody (PE)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal AV20 PE
anti-Bu-1 antibody (FITC)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal AV20 FITC
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应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
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样本量
- 1 mL
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
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储存液
- Without preservative
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注意事项
- Each reagent is stable for the period shown on the bottle label if stored as directed.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C
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: "Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." in: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).
: "The Postembryonic Development of the Immunological Barrier in the Chicken Spleens." in: Journal of immunology research, Vol. 2019, pp. 6279360, (2019) (PubMed).
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: "Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." in: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).
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- Bu-1
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背景
- Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
抗原
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