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Bu-1 抗体 (Biotin)

适用: 小鸡 FACS 宿主: 小鼠 Monoclonal AV20 Biotin
产品编号 ABIN135596
发货至: 中国
  • 抗原 See all Bu-1 products
    Bu-1
    适用
    小鸡
    宿主
    • 4
    小鼠
    克隆类型
    • 4
    单克隆
    标记
    • 2
    • 1
    • 1
    This Bu-1 antibody is conjugated to Biotin
    应用范围
    • 4
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    特异性
    Chicken Bu-1, Mr 70 kDa
    产品特性
    Mouse Anti-Chicken Bu-1-BIOT
    免疫原
    Chicken peripheral blood lymphocytes and splenocytes
    克隆位点
    AV20
    亚型
    IgG1
  • 应用备注
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    样本量
    1 mL
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    0.5 mg in 1.0 mL of PBS/Sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C
  • Mucksová, Plachý, Staněk, Hejnar, Kalina, Benešová, Trefil: "Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." in: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).

  • 抗原
    Bu-1
    Abstract
    Bu-1 产品
    别名
    B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody
    背景
    Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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