Phosphotyrosine 抗体 (HRP)
Our Local Distributor
北京 101111
Quick Overview for Phosphotyrosine 抗体 (HRP) (ABIN135060)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
-
-
产品特性
- The antibody detects: Phosphorylated tyrosine and phenylphosphate
-
纯化方法
- Purified
-
亚型
- IgG2b
-
-
anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 390
-
-
应用备注
-
Working Dilution:
ELISA: 1:1,000-1:4,000
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. -
样本量
- 1 mL
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.
-
注意事项
-
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
储存条件
- 4 °C
-
-
- Phosphotyrosine
-
物质类
- Amino Acid
-
背景
- Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
抗原
-
