BrdU 抗体 (PE)
(8 references)Quick Overview for BrdU 抗体 (PE) (ABIN1303172)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-BrdU PE
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特异性
- The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Bromodeoxyuridine conjugated to BSA
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亚型
- IgG1
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, FACS, IF, WB, ELISA, FM, DB 宿主: 小鼠 Monoclonal 29G6-E8 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), ICC, IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
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限制
- 仅限研究用
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 2, pp. H944-53, (2008) (PubMed).
: "CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair." in: Bone marrow transplantation, Vol. 41, Issue 11, pp. 983-9, (2008) (PubMed).
: "Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 30, pp. 7939-53, (2007) (PubMed).
: "Critical requirement for cell cycle inhibitors in sustaining nonproliferative states." in: The Journal of cell biology, Vol. 176, Issue 6, pp. 807-18, (2007) (PubMed).
: "Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, Vol. 29, Issue 1, pp. 33-8, (2005) (PubMed).
: "Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine." in: American journal of physiology. Cell physiology, Vol. 289, Issue 3, pp. C757-65, (2005) (PubMed).
: "Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 12, pp. 1681-8, (2003) (PubMed).
: "Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 10, pp. 1517-27, (1989) (PubMed).
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: "Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 2, pp. H944-53, (2008) (PubMed).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU
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物质类
- Chemical
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背景
- Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.,5-bromodeoxyuridine
抗原
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