Nitrotyrosine 抗体
Quick Overview for Nitrotyrosine 抗体 (ABIN1302502)
抗原
See all Nitrotyrosine 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Nitrotyrosine Purified
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特异性
- The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
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交叉反应 （详细）
- Broad
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- NO2-Tyr-CH2-Thyroglobulin
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亚型
- IgG1
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anti-Nitrotyrosine antibody
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 unconjugated
anti-Nitrotyrosine antibody (HRP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 HRP
anti-Nitrotyrosine antibody (FITC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 FITC
anti-Nitrotyrosine antibody (Biotin)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Biotin
anti-Nitrotyrosine antibody (PE)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PE
anti-Nitrotyrosine antibody (PerCP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PerCP
anti-Nitrotyrosine antibody (APC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC
anti-Nitrotyrosine antibody (Atto 594)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 594
anti-Nitrotyrosine antibody (Atto 488)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 488
anti-Nitrotyrosine antibody (Atto 390)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 390
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- Nitrotyrosine
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物质类
- Chemical
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背景
- Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
抗原
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