MEK1/2 抗体
Quick Overview for MEK1/2 抗体 (ABIN1302344)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-MEK1/2 Purified
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特异性
- The polyclonal antibody reacts with intracellular antigens MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.
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纯化方法
- Purified by ligand affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (AA 301-393) antibody
MAP2K1/2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (pSer218), (pSer222) antibodyMAP2K1/2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (pSer218), (pSer222) antibodyMAP2K1/2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (Ser112) antibodyMAP2K1/2 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (AA 193-242), (pSer222) antibodyMAP2K1/2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2) (AA 189-238), (pSer218) antibodyMAP2K1/2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blotting: Recommended dilution 0.5 μg/mL, positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))
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别名
- MEK1/2
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背景
- MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade –, an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.,MAPKK 1/2, MKK1/2
抗原
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