GLI2 抗体 (Internal Region)
(1 reference)
Quick Overview for GLI2 抗体 (Internal Region) (ABIN129624)
抗原
See all GLI2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-695
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Supplier
- Rockland
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原理
- Gli2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against mouse Gli-2 protein.
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产品特性
- Synonyms: rabbit anti-Gli-2 antibody, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein antibody, Thp antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody
GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1287-1321), (C-Term) antibodyGLI2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB49147 unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (C-Term) antibodyGLI2 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 600-649) antibodyGLI2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 30-65) antibodyGLI2 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 2-20) antibodyGLI2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (N-Term) antibodyGLI2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1444-1463), (C-Term) antibodyGLI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 2 μg/mL to 20 μg/mL
Application Note: This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by WB and IHC. Multiple splice variants have been reported for this protein.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:15,000 - 1:60,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.02 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Conditional ablation of Pten in osteoprogenitors stimulates FGF signaling." in: Development (Cambridge, England), Vol. 138, Issue 7, pp. 1433-44, (2011) (PubMed).
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: "Conditional ablation of Pten in osteoprogenitors stimulates FGF signaling." in: Development (Cambridge, England), Vol. 138, Issue 7, pp. 1433-44, (2011) (PubMed).
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- GLI2 (GLI Family Zinc Finger 2 (GLI2))
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别名
- Gli2
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背景
- Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, and postaxial polydactyly types A1 and B. Anti-Gli 2 Antibody is useful for researchers interested in transcription factor activities, DNA binding, and chromatin binding research.
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基因ID
- 14633, 124487481
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UniProt
- Q8K0K3
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis
抗原
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