SETDB1 抗体 (AA 1050-1075)
(4 references)
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Quick Overview for SETDB1 抗体 (AA 1050-1075) (ABIN129618)
抗原
See all SETDB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1050-1075
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Supplier Product No.
- 600-401-689
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Supplier
- Rockland
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原理
- ESET Antibody
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交叉反应 （详细）
- Reactivity occurs against human ESET protein.
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产品特性
- Synonyms: rabbit anti-ESET antibody, ERG-associated protein with SET domain, rabbit anti-SETDB1 antibody, SETDB-1, SETDB 1, Histone lysine N methyltransferase antibody, Histone-lysine N-methyltransferase SETDB1 antibody, KMT1E antibody, Lysine N-methyltransferase 1E antibody, SET domain bifurcated 1, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4
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纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-100) antibody
SETDB1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4A3 unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1-397) antibodySETDB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) antibodySETDB1 适用: 人, 小鼠, 猴 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 5H6A12 unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1-397) antibodySETDB1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) antibodySETDB1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1193-1225) antibodySETDB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-100) antibodySETDB1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1193-1225), (C-Term) antibodySETDB1 适用: 人 WB 宿主: 兔 Polyclonal RB5748 unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:1,000 - 1:2,500
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.17 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, (2004) (PubMed).
: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, (2003) (PubMed).
: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, (2002) (PubMed).
: "Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 83-6, (1999) (PubMed).
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: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, (2004) (PubMed).
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- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
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别名
- SETDB1
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背景
- Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.
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基因ID
- 9869, 224177469
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UniProt
- Q15047
抗原
-
