RAD52 抗体 (AA 350-375)
Quick Overview for RAD52 抗体 (AA 350-375) (ABIN129613)
抗原
See all RAD52 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 350-375
-
Supplier Product No.
- 600-401-679
-
Supplier
- Rockland
-
原理
- RAD52 Antibody
-
交叉反应 （详细）
- Reactivity occurs against human RAD52 protein.
-
产品特性
- Synonyms: rabbit anti-RAD52 antibody, RAD-52, RAD 52, DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog antibody, recombination protein RAD52 antibody
-
纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 269-418) antibody
RAD52 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5E11E7 unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Internal Region) antibodyRAD52 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Thr56) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 125-418) antibodyRAD52 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119), (pTyr104) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Thr56) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: This affinity purified antibody has been tested by WB and ELISA. Anti-RAD52 is useful in western blotting using HeLa nuclear extracts. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
-
别名
- RAD52
-
背景
- Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).
-
基因ID
- 5893
-
NCBI登录号
- NP_602296
-
UniProt
- P43351
-
途径
- DNA Damage Repair
抗原
-
