WRNIP1 抗体 (Internal Region)
Quick Overview for WRNIP1 抗体 (Internal Region) (ABIN129611)
抗原
See all WRNIP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-676
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Supplier
- Rockland
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原理
- WHIP Antibody
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交叉反应 （详细）
- Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.
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产品特性
- Synonyms: rabbit anti-WHIP antibody, ATPase WRNIP 1 antibody, ATPase WRNIP1 antibody, Werner helicasae interacting protein 1 antibody, WRNIP-1
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纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Werner Helicase Interacting Protein 1 (WRNIP1) (AA 67-116) antibody
WRNIP1 适用: 人, 小鼠, 大鼠, Cow, 猴, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) (N-Term) antibodyWRNIP1 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) antibodyWRNIP1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) (C-Term) antibodyVerified WRNIP1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) (AA 403-665) antibodyWRNIP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) (C-Term) antibodyWRNIP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Werner Helicase Interacting Protein 1 (WRNIP1) (N-Term) antibodyWRNIP1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa). Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- WRNIP1 (Werner Helicase Interacting Protein 1 (WRNIP1))
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别名
- WRNIP1
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背景
- Background: Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).
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基因ID
- 56897, 55661735
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UniProt
- Q96S55
抗原
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