ATF3 抗体 (Internal Region)
(1 reference)
Quick Overview for ATF3 抗体 (Internal Region) (ABIN129602)
抗原
See all ATF3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-493
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Supplier
- Rockland
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原理
- ATF3 Antibody
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交叉反应 （详细）
- This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems.
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产品特性
- Synonyms: rabbit anti-ATF3 Antibody, ATF 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3
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纯化方法
- Affinity purified antibody
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过滤
- Sterile filtered
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免疫原
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibody
ATF3 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7F1B10 unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 51-150) antibodyATF3 适用: 人, 小鼠, 大鼠 WB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibodyATF3 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6B8 unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibodyATF3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibodyATF3 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5A9B9 unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 140-168) antibodyATF3 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibodyATF3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 3 (ATF3) (AA 1-181) antibodyATF3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8D8 unconjugated
anti-Activating Transcription Factor 3 (ATF3) (C-Term) antibodyATF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 3 (ATF3) antibodyATF3 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal 44C3a unconjugated
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应用备注
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Application Note: Affinity purified anti-ATF3 has been tested by ELISA and western blotting against recombinant forms of the protein. Although not tested, this antibody is likely function in most immunoassays including immunofluorescence microscopy, immunohistochemistry.
Western Blot Dilution: 1:200 - 1:2000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells." in: The American journal of pathology, Vol. 176, Issue 2, pp. 939-51, (2010) (PubMed).
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: "Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells." in: The American journal of pathology, Vol. 176, Issue 2, pp. 939-51, (2010) (PubMed).
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- ATF3 (Activating Transcription Factor 3 (ATF3))
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别名
- ATF3
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背景
- Background: ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.
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基因ID
- 467
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NCBI登录号
- NP_001025458
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UniProt
- P18847
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途径
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
抗原
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