PNKP 抗体 (Internal Region)
Quick Overview for PNKP 抗体 (Internal Region) (ABIN129595)
抗原
See all PNKP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-480
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Supplier
- Rockland
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原理
- PNK Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human PNK protein.
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产品特性
- Synonyms: rabbit anti-PNKP antibody, rabbit anti PNK antibody, rabbit anti-polynucleotide kinase antibody, Bifunctional polynucleotide phosphatase/kinase, Mammalian PNK, PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 60-320) antibody
PNKP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) antibodyPNKP 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) antibodyPNKP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 359-408) antibodyPNKP 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人, 小鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-243) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 109-138), (N-Term) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to PNK by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:300 - 1:1,500
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.46 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
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别名
- PNKP
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背景
- Background: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.
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基因ID
- 11284, 31543419
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UniProt
- Q96T60
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途径
- DNA Damage Repair, Nucleotide Phosphorylation
抗原
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