SFRP1 抗体
(3 references)
Quick Overview for SFRP1 抗体 (ABIN129592)
抗原
See all SFRP1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-475
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Supplier
- Rockland
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原理
- SFRP1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human SFRP protein.
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产品特性
- Synonyms: rabbit anti-SFRP1 antibody, SFRP-1, secreted frizzled related protein 1 antibody, FRP-1 antibody, FRP 1 antibody, FRP1 antibody, FrzA antibody, SARP 2 antibody, SARP2 antibody, Secreted Apoptosis-related Protein 2 antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: SFRP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 8-153) antibody
SFRP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 201-314) antibodySFRP1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 170-240) antibodySFRP1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 26-55), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB21557 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 30-314) antibodySFRP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 33-63), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) antibodySFRP1 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:800
Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and IHC. This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200-1:2,000
ELISA Dilution: 1:5,000 - 1:15,000
IF Microscopy Dilution: Use at a concentration of 5 μg/mL
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma." in: BMC cancer, Vol. 7, pp. 126, (2007) (PubMed).
: "Evaluation of SFRP1 as a candidate for human retinal dystrophies." in: Molecular vision, Vol. 10, pp. 426-31, (2004) (PubMed).
: "Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2832-40, (2004) (PubMed).
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: "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma." in: BMC cancer, Vol. 7, pp. 126, (2007) (PubMed).
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- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
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别名
- SFRP1
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背景
- Background: Anti-SFRP1 is a Stem Cell Antibody. SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.
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基因ID
- 6422, 56117838
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UniProt
- Q8N474
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
抗原
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