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Aurora Kinase B 抗体 (AA 225-250)

There is 1 publication for this product available. The 兔 多克隆 anti-Aurora Kinase B antibody is suitable to detect Aurora Kinase B in samples from 人. It has been validated for WB, ELISA 和 FM.
Rockland
产品编号 ABIN129590
Supplier Product No.: 600-401-465
发货至: 中国
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Quick Overview for Aurora Kinase B 抗体 (AA 225-250) (ABIN129590)

抗原

See all Aurora Kinase B (AURKB) 抗体
Aurora Kinase B (AURKB)

适用

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多克隆

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This Aurora Kinase B antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
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    AA 225-250

    Supplier Product No.

    600-401-465

    Supplier

    Rockland

    原理

    AURORA KINASE B Antibody

    交叉反应 (详细)

    Reactivity occurs against human Aurora Kinase B protein.

    产品特性

    Synonyms: rabbit anti-Aurora B Antibody, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

    纯化方法

    Aurora B antibody is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

    过滤

    Sterile filtered

    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 225-250 of Human Aurora Kinase B protein.

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Application Note: Anti-Aurora B is affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.   Human brain tissue lysate or HeLa cell lysate can be used as a positive control.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:5,000 - 1:20,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.39 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Anti-Aurora B at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Slawson, Lakshmanan, Knapp, Hart: "A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4130-40, (2008) (PubMed).

  • 抗原

    Aurora Kinase B (AURKB)

    别名

    AURKB

    背景

    Background: Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body.  High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

    基因ID

    9212, 83776600

    UniProt

    Q96GD4

    途径

    TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades
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