POLM 抗体 (AA 6-18)
Quick Overview for POLM 抗体 (AA 6-18) (ABIN129588)
抗原
See all POLM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 6-18
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Supplier Product No.
- 600-401-462
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Supplier
- Rockland
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原理
- POLM Mu Antibody
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产品特性
- Synonyms: rabbit anti-POLM antibody, DNA-directed DNA/RNA polymerase mu , Pol Mu, Terminal transferase, DNA Polymerase Mu antibody, DNA-directed DNA polymerase mu antibody, Pol iota antibody
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纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 6-18 of Human POLM m (DNA polymerase mu). POLM mu is a template-directed terminal deoxynucleotide transferase that shows a preferential expression in proliferating lymphoid cells.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Polymerase (DNA Directed), mu (POLM) (C-Term) antibody
POLM 适用: 人, 马, 小鼠, Pig, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- POLM (Polymerase (DNA Directed), mu (POLM))
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别名
- POLM
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背景
- Background: DNA-directed DNA/RNA polymerase mu (POLM) is a Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). It participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. DPOLM has a reduced ability to distinguish dNTP and rNTP as substrates, and elongates them on DNA primer strand with a similar efficiency. It is able to polymerize nucleotides on RNA primer strands.
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基因ID
- 27434
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UniProt
- Q9NP87
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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