MAD2L1 抗体 (AA 1-25)
Quick Overview for MAD2L1 抗体 (AA 1-25) (ABIN129587)
抗原
See all MAD2L1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-25
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Supplier Product No.
- 600-401-461
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Supplier
- Rockland
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原理
- MAD2L1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human MAD2L1 protein.
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产品特性
- Synonyms: rabbit anti-MAD2L1 antibody, MAD2, MAD2-like protein 1, HsMAD2, Mitotic arrest deficient 2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal region near amino acid residues 1-25 of Human MAD2L1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (C-Term) antibody
MAD2L1 适用: 人, 大鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2-1D6 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-174) antibodyMAD2L1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人 WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 17D10 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (Internal Region) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 131-205) antibodyMAD2L1 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.19 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))
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别名
- MAD2L1
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背景
- Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. This protein has a nuclear localization.
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基因ID
- 4085, 4505067
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UniProt
- Q13257
抗原
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