DNMT3L 抗体 (AA 150-175)
Quick Overview for DNMT3L 抗体 (AA 150-175) (ABIN129584)
抗原
See all DNMT3L (TRDMT1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 150-175
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Supplier Product No.
- 600-401-454
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Supplier
- Rockland
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原理
- DNMT3L Antibody
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交叉反应 （详细）
- This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76 % identity with mouse.
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产品特性
- Synonyms: rabbit anti-DNMT3L antibody, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L
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纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibody
TRDMT1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 147-386) antibodyTRDMT1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2D10H11 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) antibodyTRDMT1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (Internal Region) antibodyTRDMT1 适用: 人, 小鼠 WB, ELISA, IHC, IF, IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibodyTRDMT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-234) antibodyTRDMT1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibodyTRDMT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 357-388), (C-Term) antibodyTRDMT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB02219 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 383-417), (C-Term) antibodyTRDMT1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50995 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 258-290) antibodyTRDMT1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50994 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500
Application Note: Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide. This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation. The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1000 - 1:3000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.11 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
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别名
- DNMT3L
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背景
- Background: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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基因ID
- 29947
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NCBI登录号
- NP_787063
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UniProt
- Q9UJW3
抗原
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