RNF34 抗体 (AA 1-373)
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北京 101111
Quick Overview for RNF34 抗体 (AA 1-373) (ABIN129536)
抗原
See all RNF34 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-373
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Supplier Product No.
- 200-401-933
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Supplier
- Rockland
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原理
- RNF34 Antibody
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交叉反应 （详细）
- This protein A purified antibody is directed against human RNF34 protein.
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产品特性
- Synonyms: rabbit anti-RNF34 Antibody, rabbit anti-RNF 34 Antibody, Caspase regulator CARP1 antibody, CARP 1 antibody, hRFI antibody, RFI antibody, RIFF antibody, Ring finger protein 34 antibody
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纯化方法
- The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Ring Finger Protein 34 (RNF34) (AA 1-220) antibody
RNF34 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (AA 1-373) antibodyRNF34 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (AA 143-192) antibodyRNF34 适用: 人, Cow, 兔, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (Middle Region) antibodyRNF34 适用: 人, Cow, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:4,000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 5.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RNF34 (Ring Finger Protein 34 (RNF34))
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别名
- RNF34
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.
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基因ID
- 80196, 37595539
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UniProt
- Q969K3
抗原
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