MAD2L1 抗体
Quick Overview for MAD2L1 抗体 (ABIN129510)
抗原
See all MAD2L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-461
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Supplier
- Rockland
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原理
- MAD2L1 Antibody
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交叉反应 （详细）
- This Protein A purified antibody is directed against human MAD2L1 protein.
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产品特性
- Synonyms: mouse anti-MAD2L1 antibody, Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody
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纯化方法
- The product was purified from tissue culture supernatant by chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1
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anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (C-Term) antibody
MAD2L1 适用: 人, 大鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2-1D6 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-174) antibodyMAD2L1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人 WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 17D10 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (Internal Region) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 131-205) antibodyMAD2L1 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein A purified antibody has been tested for use in immunoprecipitation and by western blot. This antibody is not applicable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))
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别名
- MAD2L1
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背景
- Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14.
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基因ID
- 4085, 4505067
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UniProt
- Q13257
抗原
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